Millions Knives is Vash's evil twin brother and is the real hidden threat on the desert planet, Gunsmoke. It was Knives, not Vash, who was responsible for "The Great Fall." He believes that humanity is a plague that must be exterminated because they survive by consuming the plants and all the planets natural resources.

Brilliant Dynamites Neon is the leader of a large gang of armed bandits known as the Bad Lads. He's an enormous man, bulging with muscles and wearing clothing decked out with neon lights covering almost every inch of his body. He encounters Vash while raiding a sandsteamer Vash happened to be traveling on, and after the ensuing chaos the two decide to have a duel, to determine the fate of the sandsteamer. After a brief exchange of gunfire, both of them walk away damaged from the duel, though Vash escaped with no wounds inflicted by Neon, but by a member of the Badlads that had shot Vash earlier in the episode when he hesitated to shoot to kill. The wound had opened up during the duel, and had resulted in the draw.

Legato Bluesummers loves death, and devotes his life to making Vash the Stampede experience eternal pain and suffering. He also wears a coat, making him synonnymous with the japanese color of death, white. He is a modified human and the leader of the Gung-Ho Guns. He was recruited by Millions Knives after the July incident to make Vash's life a living hell. His strong resonance with Vash actually stems from the fact that Vash's left arm is grafted onto him. Legato possesses psychic powers that can force his victims to do anything he wishes through telepathic control. Legato forces Vash to kill him by using his powers to threaten Milly and Meryl. Legato thus becomes the first person that Vash ever consciously and willfully killed, fulfilling his promise to "make Vash the Stampede suffer eternal pain".